Contamination is the WORST

My update today is about how contamination is sad and throws terrible wrenches into very tight timelines. I am referring to a natural transformation I have been attempting to perform to get a particular cag pathogenicity island plasmid into H. pylori. Unfortunately, we had a bad batch of plates and they all came up contaminated very quickly (probably with some Staph strain) and made our incubator smell horrendous. This procedure takes about two weeks from start to finish, and that’s before experiments, so it’s looking like I might not be able to include that data in my thesis, but fingers crossed that things start to pick up with less contamination!

In the meantime, I have been doing a lot of data analysis and writing, and it looks like my story about OipA and the cag pathogenicity island is coming together! Strange to think I will have a final product in just a few short weeks!