Feb
26

Results and Presentations

This past month, I have focused on finishing data collection for the biofilm/toxin competition assay I conducted last month. After processing the rest of my stored colonies, I calculated initial and final relative frequencies of the biofilm-forming strains in the assay and visualized these data graphically. The resulting graphs illustrate clearly that toxin production affects…
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Jan
31

Continuing Biofilm/Toxin Assays: Progress!

I have been putting a lot of time into lab so far this semester. A lot of my work has been towards generation of my Trojan Horse strain. I have done transformations with both an extrachromosomal plasmid and with a cassette targeted for integration into the yeast genome. I am now assaying potentially successful transformants…
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Dec
30

Post-Winter-Break Plans

What with finals and break, I have had to put my work in the lab on hold for a few weeks; however, this time has given me the opportunity to think about my plans for the future and specifically about what I hope to accomplish next semester.

Nov
30

November Progress

This month, I repeated my assay of toxin and biofilm interactions on four different types of media, using both K2 and K28 toxin-producing strains and two different biofilm-forming strains.

Oct
31

Moving Forward

After my last post, I finally succeeded in visualizing K28 toxin production after testing several different potentially sensitive strains. I then used these strains to test for toxin production by the strains I had previously transformed with my K28 plasmid. Sadly, the transformants did not show toxin activity. I am now changing my approach in…
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