Aug
11

Summary: End of the Summer!

Hi everyone! I cannot believe how quickly this summer of research flew by. It feels like just yesterday I was beginning my first experiment on the resistance to thyroid hormone (RTH) mutants. Although I am definitely ready to get some rest before the school year begins once again, I am very proud of the work…
Read More

Aug
07

Post #8: Aggre…what?

Aggrephagy! It’s the connection I’ve been looking for! Hi everyone! It is hard to believe that the end of this summer is rapidly approaching. However, I am very happy with all the progress I made on my thesis over the course of these past 10 weeks. Even though I am done with bench work for the…
Read More

Jul
31

Post #7: So many colors!

Hi everyone! This past week, I have been working on my co-transfection experiments with mutant tc-TRa1 and aggresome markers, GFP-170 and GFP-250. After not observing any colocalization with the autophagy marker, I didn’t know what to expect in this new round of experiments. I suspected I might see some colocalization here and there, but that…
Read More

Jul
24

Post #6: Bacteria, Transform!

Hi everyone! In my last post, I mentioned I would begin working with GFP-170 and GFP-250, well-characterized aggresome markers, to study the protein aggregation of my mutant receptor tc-TRa1. However, before I could begin those experiments, I had to create expression plasmids for tc-Tra1 that are not hybridized to GFP. After all, if both my receptor and the…
Read More

Jul
20

Post #5: Chimera Science

Hi everyone! As you might remember from my last post, I successfully expressed the autophagy marker LC3 in HeLa cells, but it did not co-localize with my mutant receptor. Since then, I have been researching different methods that I could potentially use to study the protein aggregation of mutant tc-TRa1. One particular method that I am investigating is…
Read More