Jul
20

Post #5: Chimera Science

Hi everyone! As you might remember from my last post, I successfully expressed the autophagy marker LC3 in HeLa cells, but it did not co-localize with my mutant receptor. Since then, I have been researching different methods that I could potentially use to study the protein aggregation of mutant tc-TRa1. One particular method that I am investigating is…
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Jul
11

Post #4: LC3 in Cells

Hi everyone! Over this past couple of weeks, I have been analyzing images of my co-transfections with mutant TRa1 receptor and the autophagy marker, LC3. I was very excited to see that the LC3 marker was expressing very well in HeLa cells. In some of the images I obtained (Figure 1), I could clearly see spots…
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Jul
01

Post #3: Done with RTH! Back to Oncogenesis

Hi everyone! I am very excited to share with you my results for my RTH experiments. So far, have not found a significant difference in N/C ratios between the wild-type receptor, and the mutant TRa1 receptors.

Jun
15

Post #2: Working with RTH Mutants!

Hi everyone! Since my last post, I have been working on my resistance to thyroid hormone syndrome (RTH) experiments. I expressed my two TR-a1 mutants in a human cell line, and analyzed them under fluorescence microscope. In order to compare the nuclear localization of these mutants to the wild-type receptor, I need to quantify the fluorescence in…
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Jun
03

Post #1: First Experiment Started!

Hi everyone! I am back in Dr. Allison’s lab and I have begun working on my first set of experiments this summer. With this specific experiment, I will be looking at the intracellular localization patterns of two TR-a1 mutants that have been previously linked to resistance to thyroid hormone syndrome (RTH), an autosomal recessive disorder in which…
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