Mar
27

Into the Home Stretch

After well over a year on this project, I’m starting to wrap up my research in order to finish writing my thesis. The date is officially set for my defense, and I’m tying up the loose ends nicely. I have one set of experiments left to run, which I’m hoping to finish up in the…
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Mar
13

Allosteric Regulators on Pyruvate Kinase Oxidation

Research has been going extremely well lately. The main focus of my research over the past month has been the role of fructose-1,6-bisphosphate in preventing oxidative damage to the enzyme of interest – pyruvate kinase. PK has two main binding sites with cysteines critical for function: the catalytic active site and the F-1,6-bisP regulatory site.…
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Jan
19

The Many Faces of Pyruvate Kinase

When I typically talk about my research with pyruvate kinase, I talk about the negative effects of oxidative damage on metabolism with regards to neurodegeneration. But, there are so many other ways that loss of function of pyruvate kinase when exposed to oxidation can harm the body including hemolysis and cell proliferation.

Jan
18

Making the Most of a New Semester

I can’t believe today is my last first day of classes at W&M. To kick off the semester, I thought I’d write an update for the past six weeks (December and January thus far). Break gave me a chance to relax and refocus coming into the semester. I’ve laid out my goals and a general…
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Nov
21

November Update

A lot has happened these few weeks, and I’m excited to move forward with these last sessions of lab for the semester. Last time, I talked about oxidizing pyruvate kinase with DTNB – a cysteine-specific reagent. We’ve oxidized PK with HOCl and seen an inhibition of activity, but we don’t know what exactly is getting…
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