Mar
30

Final Stretch!

This month, I succeeded in transforming three strains to express the K2 killer toxin on an extrachromosomal plasmid. I am now conducting one last experiment for the semester, competing these engineered strains with a biofilm-forming strain under different initial strain proportions. I will be photographing and processing colonies over the next few days. I am…
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Mar
27

Into the Home Stretch

After well over a year on this project, I’m starting to wrap up my research in order to finish writing my thesis. The date is officially set for my defense, and I’m tying up the loose ends nicely. I have one set of experiments left to run, which I’m hoping to finish up in the…
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Mar
25

Almost There

Hello everyone, I’m happy to tell you that I am very close to the end of data collection. I believe I am just 5 participants from reaching my goal of 80. Not bad! Ideally, we’d have more participants at this point but it’s possible that someone may continue collecting data for this project next semester. Compared to…
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Mar
21

The End?

Just over one year ago, the thesis process was beginning, specifically with this blog. This is the twelfth and penultimate post. My project is beginning to wrap up and will be officially finished within just a couple of weeks. Reflecting on my thoughts a year ago, I recall the excitement of what was to come. The crazy…
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Mar
13

Allosteric Regulators on Pyruvate Kinase Oxidation

Research has been going extremely well lately. The main focus of my research over the past month has been the role of fructose-1,6-bisphosphate in preventing oxidative damage to the enzyme of interest – pyruvate kinase. PK has two main binding sites with cysteines critical for function: the catalytic active site and the F-1,6-bisP regulatory site.…
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